The cephalic index is a head measurement technique by determines the ratio of head size from the maximum length of the skull bone (glabella-opisthocranion) to the maximum width of the skull bone (euryon-euryon). Ethnicity is one of the factors that can affect the shape and size of a person’s head. The Timorese are a tribe that lives on the island of Timor in East Nusa Tenggara Province. Intermarriage between different ethnicities can affect the next generation’s shape and type of head. This is due to the combination of different genetic information from each individual. This study aims to determine whether there is a difference in the mean cephalic index between the Timorese and the mixed Timorese in Kupang City, East Nusa Tenggara Province. This study uses a comparative analytical research design with a cross-sectional design. The sampling technique used was a non-probability sampling method with a consecutive sampling type, with a sample size of 100 people. The data were collected using a screening sheet and measuring the cephalic index directly on research subjects using a spreading caliper. The data analysis used was the independent t-test. The result showed that using an independent t-test, there was no significant difference in the mean cephalic index between the Timorese and mixed Timorese in Kupang City, East Nusa Tenggara Province with p-value = 0.38.