Psychological Linguistic Framing (PLF) introduces a first-of-its-kind cognitive–biological audit framework demonstrating that language is not passive but a direct regulator of human physiology, emotion, and behavior. PLF reveals how words, tones, and sequences act as biological levers, influencing stress responses, calming neural pathways, shaping identity, and directing social action. At the core of this framework is the PLF Law of Biological Levers, which states: Once words are applied, neutrality collapses. Every frame—lexical, tonal, rhythmic, or sequential—functions as a biological lever, directly regulating cognition, physiology, and collective behavior. This law reframes language as a regulatory force rather than symbolic abstraction, making visible how communication continuously alters human biology and collective reality. This framework unifies insights from psychology, neuroscience, linguistics, and AI ethics into a single system that can be applied across fields. It provides tools to audit harmful framing in media, politics, workplaces, and AI systems, while also offering low-cost, non-pharmaceutical mental health interventions through reframing. Key contributions include: Establishing language as a physiological regulator rather than symbolic abstraction. Demonstrating practical applications in therapy, education, crisis response, and AI safety. Exposing how propaganda, self-talk, and chatbot dialogue operate as “biological commands.” Offering preventive strategies that can be scaled globally to reduce anxiety, stress, and manipulation risks. PLF represents a foundational shift in how language is understood and used. Positioned at the intersection of mental health and artificial intelligence, it offers not only a tool for resilience and prevention but also a framework for transparency in a world increasingly shaped by language-driven systems.