Raw microscopy image from the NFDI4Bioimage calendar March 2025. The image shows 125x magnified microscopic details of a biofilm formed by Pseudomonas fluorescence on the surface of a liquid culture medium. The culture was inoculated with a cellulose-overexpressing and surface-colonizing mScarlet-tagged wild type and a GFP-tagged mutant that is unable to colonize the surface. The biofilm can collapse over time due to its own mass, so that new strategies have to be developed and thus a life cycle emerges. Image Metadata (using REMBI template): Study Study description Biofilm formation Study Component Imaging method Stereo microscopy Biosample Biological entity Bacteria Organism Pseudomonas fluorescence Specimen Signal/contrast mechanism Relief, fluorescence Channel 1 - content Relief, grey Channel 1 - biological entity Details of the biofilm in transmitted light Channel 2 - content mScarlet, red Channel 2 - biological entity WT over-expressing cellulose and colonizing the surface Channel 3 - content GFP, green Channel 3 - biological entity ∆wss mutant unable to colonize the surface Image Acquisition Microscope model Zeiss Axio Zoom V16 Image Data Magnification 125x Objective PlanNeoFluar Z 1.0x Dimension extents x: 2752, y: 2208 Pixel size description 0.91 µm x 0.91 µm Image area 2500µm x 2500µm Submitted via NFDI4BIOIMAGE