Here are the test data, containing six samples of different conditions, for bulk RNA-seq quantification pipeline 2025. Format Library Type Phred Encoding Species Number of Reads Preparation sample1 FASTQ Paired-end Phred+33 human 19,410,373 Downsampled from real data sample2 FASTQ Single-end Phred+33 mouse 16,005,450 Downsampled from real data sample3 FASTQ, multiple lanes Paired-end Phred+33 human 19,410,373 Split by lane from sample1 sample4 FASTQ, multiple lanes Single-end Phred+33 mouse 16,005,450 Split by lane from sample2 sample5 BAM/SAM (to genome) Paired-end Phred+33 human 13,856,075 Downsampled from real data sample6 BAM/SAM (to transcriptome) Single-end Phred+33 mouse 13,533,162 Downsampled from real data