The dataset contains bio-chemical data collected in South-Western Black Sea withing the Turkish EEZ in September 2021 in framework of the R/V Bilim-2 cruise supported by EU-funded BRIDGE-BS project. Discrete water samples were taken with Niskin bottles (12 L capacity) at depths, that were selected according to water density profiles. The CTD rosette with Niskin bottles was kept for at least 2 minutes at each sampling depth. The samples were further analyzed in the wet lab onboard for dissolved oxygen (by Winkler) and pH (by pH meter), and in METU-IMS lab for nutrients (by autoanalyzer) and chlorophyl parameters (by spectrometry). The data file in ODV format contains data from 25 stations on the following parameters: Dissolved Oxygen (Winkler), NH4, NO2-N, NO3-N+NO2-N, PO4, TP, Si, POC, PON, TSS, Total Chl-a, Active Chl-a, Phaeophytin, pH, Secchi disk.