The hospitality industry stands at a crossroads between a series of choices that will shape its future. Traditionally, sustainability efforts have centred on minimising environmental impact. However, an innovative approach is required for hotels to become agents of positive change. This extends from the improvement and restoration of destinations to the protection of their natural and social ecosystems. Regenerative Hospitality represents a fundamental shift in mindsets. Instead of simply reducing harm, forward-thinking hospitality businesses are now creating net-positive impacts, leaving places better than they found them. This isn't just environmental stewardship; it's a complete reimagining of business purpose within the interconnected living systems. Thanks to a series of research projects, we designed a comprehensive and structured methodology to support the transition of hospitality businesses to Regeneration by designing net-positive initiatives. The ultimate goal is to arrive at a better understanding of our interconnected living systems.