Background: Pharmaceutical analysis plays a vital role in ensuring the quality, safety, and efficacy of drugs. UV-Visible spectroscopy, being simple, rapid, and economical, is widely used for routine drug estimation. This study aimed to develop and validate a UV spectrophotometric method for the estimation of tinidazole in bulk and tablet dosage forms. Method: Tinidazole was dissolved in methanol, which was selected as the suitable solvent. The λmax was determined at 282 nm. Calibration curves were prepared in the concentration range of 20–100 μg/mL. The developed method was validated according to ICH guidelines for linearity, precision, accuracy, robustness, LOD, and LOQ. Results: Tinidazole exhibited good linearity with regression equation y = 0.074x + 0.1209 and correlation coefficient (R² = 0.9961). The method showed excellent precision with %RSD < 2% for intra-day and inter-day studies. The LOD and LOQ were found to be 0.8 μg/mL and 2.43 μg/mL, respectively. Accuracy studies revealed recovery values close to 100%, while robustness confirmed reliability. The assay of marketed tablets showed drug content of 99.84%. Conclusion: The developed UV spectrophotometric method is simple, accurate, precise, robust, and cost-effective. It is suitable for routine quality control and quantitative analysis of tinidazole in pharmaceutical formulations, offering a reliable alternative to more complex techniques such as HPLC.