Image from the NFDI4BIOIMAGE Calendar December 2025. The microscopic image shows yeast cells of the fungal model Ustilago maydis that produce single cell oil at nitrogen-starvation conditions. The genetically engineered cells are packed with oil droplets that were visualized by BODIPY staining. The study was conducted in the framework of the BioSC project "NextVegOil". Image Metadata (using REMBI template): Study Study type Visualisation of microbial oil in the fungus Ustilago maydis Study Component Imaging method Wide field whole organism microscopy Biosample Biological entity Ustilago maydis Organism Yeast cells (sporidia) Identity Ustilago maydis MB215 cyp1Δemt1Δ (published in https://doi.org/10.1128/AEM.71.6.3033-3040.2005) Intrinsic variable Glycolipid production has been ablated by genetic engineering Extrinsic variable BODIPY (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene 493/503) staining Experimental variables Cultivation time Specimen Location within biosample Overview image with yeast cells from liquid culture at nitrogen-starvation condition Preparation method Living cells attached to agarose mounts Signal/contrast mechanism Differential interference contrast and fluorescence Channel 1 - content DIC Channel 1 - biological entity Intact yeast cells Channel 2 - content BODIPY 493/503 Channel 2 - biological entity Intracellular lipid droplets Image acquisition Instrument attributes Zeiss Axio Observer.Z1; Prime BSI express; solid-state laser 488 nm Submitted via NFDI4BIOIMAGE