Image from the NFDI4BIOIMAGE Calendar January 2025. A Heart for Redox Biology: The image of primary bone mesenchymal stromal/stem cells (hBM-MSCs) was captured in a study evaluating the cellular effects of therapeutic oxidation in the context of regenerative medicine. The cells were isolated from an arthroplasty patient cohort in a joint research project between the Center for Orthopaedics at University Medical Center and the group ZIK plasmatis at the Leibniz Institute for Plasma Science and Technology (INP) in Greifswald. You can appreciate the characteristic morphology and complex actin cytoskeleton that is crucial for the cellular function of hBM-MSCs. Can you spot the heart that is formed by the prominent actin protrusions of interconnected cells? Image Metadata (using REMBI template): Study Component Imaging method Spinning-disc confocal mode, epifluorescence Biosample Biological entity Bone marrow-mesenchymal stem cells (BM-MSCs) Organism Homo sapiens Specimen Preparation method Fixation (4% PFA) Signal/contrast mechanism Fluorescence Channel 1 – content 4',6-Diamidin-2-phenylindol (DAPI; Thermo Fisher, USA), blue Channel 1 – biological entity Nuclei Channel 2 – content MitoSpy Green (Biolegend, USA), green Channel 2 – biological entity Mitochondria Channel 3 – content Flash Phalloidin Red (Biolegend, USA), orange Channel 3 – biological entity Actin Image acquisition Microscope model Operetta CLS (PerkinElmer, USA) Image data Type Raw and processed image in comparison Magnification 20x air objective (NA = 0.8) Excitation Channel 1: 365 nm; Channel 2: 475 nm; Channel 3: 550 nm Detection Channel 1: 465 nm; Channel 2: 525 nm; Channel 3: 610 nm Analysed data Image processing method Algorithm-based, unsupervised image segmentation with Harmony 4.9 analysis software (PerkinElmer, USA) Submitted via NFDI4BIOIMAGE