John 20:11–18 presents one of the most significant post-resurrection encounters in the New Testament, where Mary Magdalene becomes the first witness and messenger of the risen Christ. This paper offers a missiological reading of the passage, exploring Mary’s role as a paradigm for breaking cultural, religious, and gender barriers in mission. In a socio-historical context where women’s testimonies were often disregarded, the risen Lord entrusts Mary with the primary proclamation of the resurrection—a task central to the Christian missionary mandate. Through exegetical analysis, the study examines the theological significance of Jesus’ commissioning words, “Go to my brothers,” as an apostolic sending that informs the Church’s mission. The paper engages with missiological scholarship to highlight how Mary’s witness exemplifies the holistic nature of mission, encompassing both proclamation of the good news and the transformation of social structures that hinder marginalized voices. By situating Mary’s experience within the broader biblical and historical tradition of women in mission, the study challenges contemporary missional practice to embrace inclusivity, recognize the Spirit’s gifting across gender lines, and reclaim the pioneering contributions of women. The findings underscore the enduring relevance of Mary Magdalene’s witness as a theological and missiological model for the global Church.