PhasorPy is an open-source Python library for the analysis of fluorescence lifetime and hyperspectral images using the phasor approach. This is the fifth alpha release of the PhasorPy library. It contains several bug fixes and improvements. The new phasor_cluster_gmm function finds clusters in phasor coordinates using a Gaussian mixture model. The phasor_filter_pawflim function applies a wavelet filter using the pawFLIM library. This release supports Python 3.11 to 3.13. Python 3.10 is no longer supported. What's Changed Bump version by @cgohlke in https://github.com/phasorpy/phasorpy/pull/183 Bug Fix for phasor_calibrate when skipping axes with harmonic axis by @bruno-pannunzio in https://github.com/phasorpy/phasorpy/pull/185 Fixes for liffile 2025.2.6 by @cgohlke in https://github.com/phasorpy/phasorpy/pull/184 Fix phasor_normalize with numpy 1.24 by @cgohlke in https://github.com/phasorpy/phasorpy/pull/186 Add pawFLIM wavelet filter for phasor coordinates by @bruno-pannunzio in https://github.com/phasorpy/phasorpy/pull/167 Add dataset from zenodo.org/records/14860228 by @cgohlke in https://github.com/phasorpy/phasorpy/pull/187 Improve support for Leica image files by @cgohlke in https://github.com/phasorpy/phasorpy/pull/188 Remove notes about early stage of development by @cgohlke in https://github.com/phasorpy/phasorpy/pull/189 Add plot_histograms function by @cgohlke in https://github.com/phasorpy/phasorpy/pull/191 Fix NaN padding in phasor_to_simfcs_referenced by @cgohlke in https://github.com/phasorpy/phasorpy/pull/192 Return more metadata from IFLI and FBD readers by @cgohlke in https://github.com/phasorpy/phasorpy/pull/193 Add more formats to file I/O tutorial by @cgohlke in https://github.com/phasorpy/phasorpy/pull/194 Fix module attribute of functions in phasorpy.io by @cgohlke in https://github.com/phasorpy/phasorpy/pull/195 Add plot_image helper function by @cgohlke in https://github.com/phasorpy/phasorpy/pull/196 Add section on other means to IO tutorial by @cgohlke in https://github.com/phasorpy/phasorpy/pull/197 Bump pypa/cibuildwheel from 2.22.0 to 2.23.0 in the github-actions group by @dependabot in https://github.com/phasorpy/phasorpy/pull/198 Improve IO tutorial by @cgohlke in https://github.com/phasorpy/phasorpy/pull/199 Fix minor issues found by GitHub Copilot by @cgohlke in https://github.com/phasorpy/phasorpy/pull/202 Move parse_skip_axis function to _utils module by @cgohlke in https://github.com/phasorpy/phasorpy/pull/203 Fix new mypy errors with numpy 2.2.4 by @cgohlke in https://github.com/phasorpy/phasorpy/pull/204 Raise minimum requirements by @cgohlke in https://github.com/phasorpy/phasorpy/pull/208 Add phasor_cluster_gmm function by @R-Blanco802 in https://github.com/phasorpy/phasorpy/pull/201 Require scikit-learn>=1.5.0 by @cgohlke in https://github.com/phasorpy/phasorpy/pull/209 Support routing channels in signal_from_sdt by @cgohlke in https://github.com/phasorpy/phasorpy/pull/210 Improve tests and documentation of phasor_cluster_gmm by @cgohlke in https://github.com/phasorpy/phasorpy/pull/211 Ignore PhasorPlot.contour cmap if colors is set by @cgohlke in https://github.com/phasorpy/phasorpy/pull/213 Bump pypa/cibuildwheel from 2.23.1 to 2.23.2 in the github-actions group by @dependabot in https://github.com/phasorpy/phasorpy/pull/214 Update Ubuntu version by @cgohlke in https://github.com/phasorpy/phasorpy/pull/219 Require Cython>=3.1.0b1 by @cgohlke in https://github.com/phasorpy/phasorpy/pull/216 Add PTU file exported from FLIM_testdata.lif with photon filter by @cgohlke in https://github.com/phasorpy/phasorpy/pull/217 Fix multi-channel FLIM LABS files containing single-channel phasors by @cgohlke in https://github.com/phasorpy/phasorpy/pull/218 Release v0.5 by @cgohlke in https://github.com/phasorpy/phasorpy/pull/220 New Contributors @R-Blanco802 made their first contribution in https://github.com/phasorpy/phasorpy/pull/201 Full Changelog: https://github.com/phasorpy/phasorpy/compare/v0.4...v0.5