Environmental stiffness regulates neuronal maturation via Piezo1-mediated TTR activity Eva Kreysing1, 2, 3,*, Hélène Gautier1, Sudipta Mukherjee1,2, 3,,Katrin A Mooslehner1, 2, 3, Leila Muresan1,4, Daniel Haarhoff5, Xiaohui Zhao6, Alexander K Winkel1, Tina Borič2, 3, Sebastián Vásquez-Sepúlveda2, 3, Niklas Gampl2, 3, Andrea Dimitracopoulos1, Eva K. Pillai1, 7,8, Robert Humphrey1, 9, Ragnhildur Thóra Káradóttir9, *, and Kristian Franze1, 2, 3,* 1 Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK 2 Institute of Medical Physics and Microtissue Engineering, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestraße 91, 91052 Erlangen, Germany 3 Max-Planck-Zentrum für Physik und Medizin, Kussmaulallee 2, 91054 Erlangen, Germany 4 School of Computing and Information Science, Faculty of Science and Engineering, Anglia Ruskin University, CB1 1PT Cambridge, UK 5 Makespace Cambridge Ltd, 16 Mill Lane, Cambridge, CB2 1RX, UK 6 Department of Medicine, Cardiovascular Division, University of Cambridge, Heart and Lung Research Institute, CB2 0BB, Cambridge, UK 7 Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstraße 1, 69117 Heidelberg, Germany; 8 Developmental Biology Unit, European Molecular Biology Laboratory, Meyerhofstraße 1, 69117 Heidelberg, Germany. 9 Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge Biomedical Campus, Puddicombe Way, Cambridge CB2 0AW, UK * Correspondence: Eva Kreysing (emk42@cam.ac.uk), Ragnhildur Thóra Káradóttir (rk385@cam.ac.uk), Kristian Franze (kf284@cam.ac.uk)