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Petroleum ether and benzene leaf extracts of an endangered medicinal plant Bryonia laciniosa L was screened for primary and secondary metabolites and GC-MS profiling. Phytochemical screening of benzene and petroleum ether solvent extracts of Bryonia leaf showed the presence of Carbohydrates, proteins and amino acids, alkaloids, anthraquinones, flavonoids, glycosides, phenols, tannins, and triterpenoids. Both the extracts showed negative response to saponin and volatile oil. The petroleum extract showed Positive response to steroids and sterols and benzene extract did not respond to steroids and sterols. GC- MS analysis identified 25 biologically active constituents present in the ethanolic extract of leaves of Bryonia laciniosa.