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ZENODO
Article . 2015
License: CC 0
Data sources: Datacite
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ZENODO
Article . 2015
License: CC 0
Data sources: ZENODO
Claim

The Electrophoretic Mobility Shift Assay (Emsa)

descriptionPublicationkeyboard_double_arrow_right Article 13 Apr 2015Publisher:Zenodo
Authors: sprotocols;

doi: 10.5281/zenodo.16778

The Electrophoretic Mobility Shift Assay (Emsa)

Abstract

The electrophoretic mobility shift assay (EMSA), also known as “gel shift assay”, is used to examine the binding parameters and relative affinities of protein and DNA interactions. We produced recombinant CCA1 protein and tested its binding affinity for the promoter fragments that contain CBS (AAAAATCT) or evening element (EE, AAAATATCT) (1) using a modified procedure adopted from published protocols (2,3).

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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