This research to get the optimal solution for the treatment of wastewater for Labanita dairy factory, which lies in Noubariya City, Behira Governorate, Egypt. A pilot lab scale was erected in the Labanita dairy factory laboratory. The pilot consisted from feeding tank followed by DAF unit followed by SBR unit. Different operation scenarios had been applied to determine the optimal operation procedure and the best removal efficiency. Samples were taken continuously for 3 months from the inlet, outlet and between the units of treatment to determine the parameters (COD, TSS, TDS and pH), in order to get the removal efficiency for each unit as well as the overall efficiency. The study illustrated the need of aeration system to be continuous that improved the dissolved oxygen stability in the SBR and improves the tank COD removal efficiency. The decreasing of chemical dose for soda or acid to 10% its used value not affected the DAF removal efficiency but improved the plant removal efficiency for COD & TDS and decrease the running cost for the minimization of chemical’s needs. The increasing of inflow after the fixation of the previous items achieved the required efficiency with 150% of the startup flow due to known design criteria. The decrease of aeration period in the SBR unit to 5 hours instead of 6 hours had achieved the higher removal efficiency for COD, TSS & TDS.