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scProAtlas: an atlas of multiplexed single-cell spatial proteomics imaging in human tissues

Authors: Wang, Tiangang;

scProAtlas: an atlas of multiplexed single-cell spatial proteomics imaging in human tissues

Abstract

All analysis results for the spatial proteomics imaging techniques in the scProAtlas database are stored in compressed files named accordingly. Within each compressed file, the folders are organized in a fixed storage structure in the following order: Analysis module > Imaging Technique > Dataset > Tissue > ROI. Each folder contains the corresponding metadata (including original sample information, cell type annotations, and neighborhood annotations) stored in a file named cells.tsv. Additionally, the module used to identify spatial pattern genes includes an anndata format file, named adata_moran.h5ad, which stores the integrated results of scRNA-seq and spatial proteomics. scProAtlas_analysis_code.tar.gz contains example codes for all analysis modules in scProAtlas. Here, we provide the example using SCP_CODEX1 - Large intestine. The codes include all the scripts used for the entire workflow, from image segmentation to scRNA-spatial proteomics integration, and spatial analysis. We have also uploaded the raw protein channel matrices with AnnData format in version 3 and 4.

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Keywords

spatial proteomics

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average