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ZENODO
Article . 2024
License: CC BY
Data sources: ZENODO
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ZENODO
Article . 2025
License: CC BY
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Article . 2025
License: CC BY
Data sources: Datacite
ZENODO
Article . 2024
License: CC BY
Data sources: Datacite
ZENODO
Article . 2025
License: CC BY
Data sources: Datacite
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A guide to selecting high-performing antibodies for GPNMB (UniProt ID: Q14956) for use in western blot, immunoprecipitation, and immunofluorescence

descriptionPublicationkeyboard_double_arrow_right Article 11 Dec 2024Publisher:Zenodo
Authors: Ayoubi, Riham; Alende, Charles; Fotouhi, Maryam; González Bolívar, Sara; Ruiz Moleon, Vera; Laflamme, Carl;

doi: 10.5281/zenodo.14393181 , 10.5281/zenodo.14393182 , 10.5281/zenodo.16543752

A guide to selecting high-performing antibodies for GPNMB (UniProt ID: Q14956) for use in western blot, immunoprecipitation, and immunofluorescence

Abstract

This report provides a guide for selecting high-quality commercial antibodies against the transmembrane glycoprotein NMB (GPNMB) for applications in western blot, immunoprecipitation, and immunofluorescence, using a knockdown cell line as an isogenic control. This research was partially supported by the Emory-Sage-SGC TREAT-AD center, established by the National Institutes of Aging under award number U54AG065187.

Keywords

Transmembrane glycoprotein NMB, Hematopoietic growth factor inducible neurokinin-1 type, antibody characterization, Q14956, antibody validation, Alzheimer's disease, GPNMB

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green