A first attempt to date the Kastrouli settlement at Desfina (Delphi Phokis) has been made by optical luminescence dating (OSL) on three ceramic and radiocarbon (C-14) of one bone sample. An initial archaeological reconnaissance of the partially looted site has produced some indication of use during late Helladic and later periods. Our four ages by luminescence and C-14 have shown that this site was used initially in Late Helladic period, and reused during the Middle Geometric, the Early Archaic and the Classical periods.