Introduction: Human papillomavirus (HPV) comprises over 200 related viruses, with high-risk types like HPV-16 and HPV-18 accounting for about 70% of cervical cancer cases—the fourth most common cancer in women globally. Materials and Methods: A cross-sectional descriptive study was conducted from September 2024 to February 2025 in the Paediatric OPD of KIMS & RF, targeting adolescent girls aged 12–15 years. The study included all girls within this age group who attended the OPD and provided consent, either personally or through a guardian. Result: The study sample consisted of 150 adolescent girls aged between 12 and 15 years, and data were collected on their sociodemographic characteristics, as well as their knowledge and attitude regarding HPV vaccination. Among the sociodemographic factors, 13-14 years age group with 34.6% followed by 14-15 years age group with 33.3%. About the knowledge of HPV infection, Cervical cancer and HPV vaccination, majority of participantsdon’t know relevant questions. Conclusion: The study concludes that inadequate knowledge and negative attitudes toward cervical cancer, HPV infection, and the HPV vaccine among adolescent girls.