We report the synthesis, characterization, thermal and microbial properties of a series of [4-N-Phenylethanamide azo-3-N-(4-Bromophenyl)Maleimide-co-Acrylonitrile] [C1-PAMI-B], [4-N-Phenylethanamide azo-3-N-(4-Bromophenyl)Maleimide-co-Acrylic acid] [C2-PAMI-B] , [4-N-Phenylethanamide azo-3-N-(4-Bromophenyl)Maleimide-co-Vinyl acetate] [C3-PAMI-B] copolymers and [4-N-Phenylethanamideazo-3-N-(4-Bromophenyl)Maleimide – Acrylonitrile - Vinyl acetate] [T1-PAMI-B] and terpolymer [4-N-Phenylethanamideazo-3-N-(4-Bromophenyl)Maleimide – Acrylic acid - Vinyl acetate] [T2-PAMI-B] terpolymers of maleimide containing compounds. Polymer’s structures of different side groups with same chain back-bone of maleimide are performed for the study of effects on glass transition point along with chain-flexibility. All polymer’s structure are confirmed by C/H/N-elemental analysis, FT-IR and 1HNMR spectral analysis. Molecular weight and polydispersity are determined by gel permeation chromatography (GPC). Thermal stability of polymers are investigated from thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Microbial activities of the polymers are also examined on various bacteria / fungi.