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ZENODO
Article . 2015
License: CC 0
Data sources: Datacite
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ZENODO
Article . 2015
License: CC 0
Data sources: ZENODO
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Co-Immunoprecipitation Of Platelet Factor 4 And Rantes From Human Platelets

descriptionPublicationkeyboard_double_arrow_right Article 15 Mar 2015Publisher:Zenodo
Authors: sprotocols;

doi: 10.5281/zenodo.16062

Co-Immunoprecipitation Of Platelet Factor 4 And Rantes From Human Platelets

Abstract

Chemokines are small proteins that have an important function in the trafficking of immune cells. Platelets also contain chemokines, which are stored in the alpha-granula. Activated platelets are able to deposit chemokines onto inflamed endothelium via transient rolling interactions, thereby enhancing subsequent arrest of e.g. monocytes. The chemokines RANTES/CCL5 and platelet factor 4 (PF4/CXCL4) are deposited by platelets and can form heteromers. These CCL5/CXCL4 heteromers are particularly potent as monocyte attractant. This protocol describes the precipitation of the CCL5/CXCL4 complex from platelets.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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