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ZENODO
Article . 2015
License: CC 0
Data sources: Datacite
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ZENODO
Article . 2015
License: CC 0
Data sources: ZENODO
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Preparation Of Peptidoglycan-Depleted Soluble Microbial Extract

descriptionPublicationkeyboard_double_arrow_right Article 12 Mar 2015Publisher:Zenodo
Authors: sprotocols;

doi: 10.5281/zenodo.15996

Preparation Of Peptidoglycan-Depleted Soluble Microbial Extract

Abstract

Unknown microbial ligand(s) other than peptidoglycan (PG) in the soluble microbial extract (SME) can induce Dual oxidase (DUOX) activity and generate reactive oxygen species in the Drosophila gut epithelia. We describe a simple method for the preparation of the PG-depleted SME (SME-ΔPG) by treatment with recombinant peptidoglycan recognition protein (PGRP)-LB, a PG-degrading enzyme. (SME-ΔPG) has been successfully used for dissecting the PG-independent immune signaling pathways in Drosophila gut. The (SME-ΔPG) will be a potentially useful material to dissect PG-independent host response to microbes in diverse biological systems.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
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