This dataset has been analyzed in the manuscript titled "SpaceBF: Spatial coexpression analysis using Bayesian Fused approaches in spatial omics datasets," as the third real data use case. There are three main data files, both in .rds format. The one named "Peptide_expression_in_spots.rds" contains the standardized expression of 12 peptides in 5548 spots. The file named "Spot_coordinates.rds" contains the xy-coordinates of the spots. The file named "Peptide_description.rds" contains the descriptive metadata of the peptides, i.e., their source genes and actual sequences. The file "ROI_114.pptx" contains the histology image. The R code to load and analyze the dataset is provided in the GitHub repository: https://github.com/sealx017/SpaceBF, the older version of the data is avilable at https://zenodo.org/records/15838609.