Abstract The 432 Hz indicates that vibration occurs 432 times within a single minute. The effects of frequency of 432 Hz on humans is studied here through the effects it has on EMG signals in an artificial intelligence based studies similar to the one I also conducted on frequency of 440 Hz (Ozuomba U. D., 2025) that many theorists had refused to acknowledge as a tuning standard for musical instruments over frequency of 432 Hz which they deemed to have better health benefits. Moreover, this study helps to divulge if frequency of 432 Hz changes electrical activities of human muscle just as many Igbo acoustic sound instrument such as ụbọ, ọja, ọkwa, nkelebe, ọdụ, aro, ataṅịrị, ekwe, ịgba, ọyọ, ogele, and vocal elele. Any changes in electrical activities of human muscle as a consequence of sound waves effects from any of these sound sources can mean detrimental health effects if they are bad, but if good, can mean healthy effects on stress, anxiety, depression, emotion, mood, affect, cognition, pain, immune system, socio-cultural integrations, profitable cooperation, personal growth and improvement as well as general well-being. Meditative sound healing practices involving some of these acoustic sound instruments are employed natively to aid pacification and mitigation of detrimental energies that survive on one’s detrimental actions and inactions that in turn drain and impedes their energy, vibrations, frequencies and general growth. These detrimental energies are seemingly karmic, and are termed akaraogheri, uke and ọgbọnuke in Igbo lingo. The sound frequency of 432 Hz at 30, 60 and 90 decibels were used for this study, and upon healthy exposure poses no health risks. As in others, outcomes may vary in diverse locations due to unwanted and unavoidable sound inputs.