Fig. 4. Analysis of variance-like differential expression plot of the most abundant (A) 16S and (B) nifH amplicon sequence variants (ASVs). X axis values are median centred-log ratio transformed abundances and y-axis values are per feature median centred-log ratio transformed abundance differences. Differences significantly different from zero are in red while those not significant are in black. Triangles are ASVs negatively loaded on PC1 in the ordinations in Fig. A2, and diamonds are positively loaded ASVs. Squares are ASVs that did not load strongly on PC1. Positive y-axis values indicate higher abundance in intact tundra and negative higher abundance in gravel substrates.

Published as part of Li, Angie S., Standen, Katherine M., Stewart, Katherine J. & Lamb, Eric G., 2025, Bacterial diversity and community structure in native legume root nodules in intact and mining-disturbed Arctic tundra, pp. 1-15 in Botany 103 (6) on page 7, DOI: 10.1139/cjb-2024-0108, http://zenodo.org/record/15809499