This is a subset of the "Denoising Tribolium" dataset, used as a training set for "AI4Life Microscopy Supervised Denoising Challenge 2025" It is based on the dataset from "Weigert, Martin, Uwe Schmidt, Tobias Boothe, Andreas Müller, Alexandr Dibrov, Akanksha Jain, Benjamin Wilhelm, et al. 2025. “Content Aware Image Restoration: Pushing the Limits of Fluorescence Microscopy Data.” Edmond. https://doi.org/doi:10.17617/3.FDFZOF. For more details about the original dataset, please see the research publication "Weigert, M., Schmidt, U., Boothe, T. et al. Content-aware image restoration: pushing the limits of fluorescence microscopy. Nat Methods 15, 1090–1097 (2018). https://doi.org/10.1038/s41592-018-0216-7"For the details about this subset and its organization, please see https://ai4life-mdc25.grand-challenge.org/leaderboard-2/The original dataset is available at https://edmond.mpg.de/dataset.xhtml?persistentId=doi:10.17617/3.FDFZOFAI4Life has received funding from the European Union’s Horizon Europe research and innovation programme under grant agreement number 101057970. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Council Executive Agency. Neither the European Union nor the granting authority can be held responsible for them. Files