A guide to selecting high-performing antibodies for TRIM21 (UniProt ID: P19474) for use in western blot, immunoprecipitation, and immunofluorescence
A guide to selecting high-performing antibodies for TRIM21 (UniProt ID: P19474) for use in western blot, immunoprecipitation, and immunofluorescence
This report provides a guide to selecting high-quality commercial antibodies against TRIM21 for use in Western blot, immunoprecipitation, and immunofluorescence, utilizing a standardized experimental protocol that compares readouts in knockout cell lines and isogenic parental controls. This work was supported by the Quebec Consortium for Drug Discovery (CQDM), a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec. RA is supported by a Mitacs fellowship.
antibody characterization, western blot, Tripartite motif-containing protein 21, P19474, antibody validation, immunofluorescence, immunoprecipitation, TRIM21, E3 ubiquitin-protein ligase TRIM21
antibody characterization, western blot, Tripartite motif-containing protein 21, P19474, antibody validation, immunofluorescence, immunoprecipitation, TRIM21, E3 ubiquitin-protein ligase TRIM21
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