2nd Debrecen Online Conference on Infectious Diseases in a One Health context The One Health approach has established itself as an important tool for the investigation, surveillance and containment of infectious diseases. Human and veterinary pathogens can spread across multiple hosts and the environment, creating complex and often poorly understood transmission networks or driving evolutionary events that lead to the emergence or re-emergence of novel or neglected infectious diseases. These include foodborne, waterborne and vector-borne diseases, as well as the hidden epidemic of antimicrobial resistance – all of which pose a major challenge to healthcare, the economy and society and require urgent attention. The 1st DOCIDOH brought together physicians, veterinarians, environmentalists, epidemiologists, molecular biologists, ecologists and various other experts to discuss issues related to One Health. As a follow-up to the first DOCIDOH and to promote discussion and knowledge exchange, the One Health Institute of the Faculty of Health Sciences, the Faculty of Agricultural and Food Sciences and Environmental Management and the Department of Metagenomics of the University of Debrecen announces the second Debrecen Online Conference on Infectious Diseases in a One Health context (DOCIDOH), which will take place from 18 to 20 June 2025. The conference aims at attracting researchers in the topics: antimicrobial resistance in a One Health perspective alternatives to antimicrobials in human and veterinary medicine genomic epidemiology of pathogens across sectors emerging and re-emerging diseases and vectors parasites ecology ecology of infections, environmental DNA and environmental health bioinformatic tools for One Health research food safety and foodborne diseases social perceptions of antibiotics and their alternatives (e.g. phage therapy, vaccination) Organizing and scientific committee Dr. Gábor Kardos – One Health Institute, Faculty of Health Sciences, University of Debrecen Dr. Csongor Freytag – One Health Institute, Faculty of Health Sciences, University of Debrecen Dr. Dalma Papp – One Health Institute, Faculty of Health Sciences, University of Debrecen Dr. Enikő Fehér – Department of Microbiology and Infectious Diseases, University of Veterinary Medicine Budapest Dr. Eszter Kaszab – Department of Microbiology and Infectious Diseases, University of Veterinary Medicine Budapest Lilla Buzgó – One Health Institute, Faculty of Health Sciences, University of Debrecen Vivien Nagy – One Health Institute, Faculty of Health Sciences, University of Debrecen Dr. Krisztina Szarka – One Health Institute, Faculty of Health Sciences, University of Debrecen Dr. Levente Laczkó – One Health Institute, Faculty of Health Sciences, University of Debrecen Dr. Pálfyné Dr. Nóra Vass – Department of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen Dr. Renáta Knop – Department of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen Dr. Márta Vargha – National Centre for Public Health and Pharmacy; One Health Institute, Faculty of Health Sciences, University of Debrecen Flórián Sipos – Institute of Metagenomics, University of Debrecen Connference website https://konferencia.unideb.hu/en/docidoh25 Programme Date Section Type Start End Section Chair Presenter Title Day 1 2025.06.18. 8:30 9:00 Opening Orsolya Surján Szabolcs Pásztor Anita Rusinné Fedor Opening speech Semi-plenary 9:00 9:30 Antimicrobial resistance in a One Health perspective Gábor Kardos Erika Tóth Environmental monitoring of AMR in the River Danube: the missing piece of the One Health puzzle Regular talks 9:30 9:45 Andrea Szabó Barriers and possibilities: One Health concept in the Hungarian antimicrobial stewardship 9:45 10:00 Randa Bazzi The role of veterinarians in the One Health approach to antimicrobial resistance perspectives in Jordan 10:00 10:15 Isma Benmazouz Antimicrobial resistance in Wild birds: Birds as AMR reservoirs and disseminators Break 10:15 10:30 Regular talks 10:30 10:45 Antimicrobial resistance in a One Health perspective Gábor Kardos Patrik Mag Antimicrobial susceptibility of Staphylococcus pseudintermedius isolates from canine dermatitis in Hungary 10:45 11:00 Ádám Kerek Phenotypic and Genotypic Characterization of Extended-Spectrum Beta-Lactamase (ESBL) Production in Commensal Escherichia coli St 11:00 11:15 Szilveszter Csorba The role of environmental factors in the spread of antimicrobial resistance in the poulty production chain Lunchbreak 11:15 12:00 Regular talks 12:00 12:15 Antimicrobial resistance in a One Health perspective Márta Vargha Máté Farkas Antibiotic resistance patterns in relation to biosecurity measures and antibiotic use based on data from pig farms 12:15 12:30 Delia-Liliana Nicoară Antibiotic-resistant Klebsiella spp. form a Preventive Medicine Approach: a review 12:30 12:45 Balázs Seres Investigating the spread of antibiotic resistance using wastewater-based epidemiology 12:45 13:00 Lilla Buzgó Comparative genomic epidemiology of multidrug-resistant Escherichia coli within the One Health approach Break 13:00 13:15 Semi-plenary 13:15 13:45 Emerging and re-emerging diseases and vectors Nóra Vass Gianvito Lanave Decoding the viral code: 25 years of virus discovery in Bari Regular talks 13:45 14:00 Anna Pataki CRESS DNA virus surveillance in wild birds 14:00 14:15 Barbara Igriczi Prevalence and genetic characterization of porcine rotavirus A in Hungarian pig farms 14:15 14:30 Fruzsina Tóth Genomic characterization of novel protoparvoviruses in domestic ruminants in Hungary 14:30 14:45 Zalán Homonnay Isolation and whole-genome characterization of Batai virus (Orthobunyavirus) from a cattle farm in Hungary 14:45 15:00 Dóra Máté Surveillance of coronaviruses with a novel broad-spectrum detection system Break 15:00 15:15 Regular talks 15:15 15:30 Emerging and re-emerging diseases and vectors Krisztina Szarka Fatima Ezzohra Ezahedi Tracing SARS-CoV-2 Evolution in Algeria: Insights from 2020–2023 15:30 15:45 Muhammad Muhsin Umar Dengue outbreaks in northern Nigeria: Evaluating the recommended Takeda vaccine and future prevention strategies 15:45 16:00 Gabor Toth Blood donors as sentinels for genomic surveillance of West Nile virus in Germany 16:00 16:15 Boglárka Pollák Wastewater-based epidemiological surveillance of respiratory pathogens 16:15 16:30 Levente Zsichla Molecular epidemiology of the HIV-1 epidemic in Hungary until 2024 Day 2 2025.06.19. Semi-plenary 8:30 9:00 Emerging and re-emerging diseases and vectors Renáta Knop Tamara Szentivanyi The evolution of mosquito and mosquito-borne disease monitoring in Hungary: past, present, future Regular talks 9:00 9:15 Nikoletta Andrea Nagy Genome of Aedes koreicus and the importance of vector genetics 9:15 9:30 Kristína Hudáková Molecular detection of Anaplasma spp. in wild animals in Eastern Slovakia 9:30 9:45 Randa Bazzi A Comprehensive Comparative Risk Analysis of Livestock Slaughterhouse Operations in Jordan and Argentina: A One Health Perspective Break 9:45 10:00 Regular talks 10:00 10:15 Food safety and foodborne diseases Erzsébet Némedi Eszter Virág Advancing Biofungicide Strategies to Ensure Healthy Crop Production 10:15 10:30 Sarita Romani Inhibition of Botritis cinerea mycelial growth by soluble and volatile metabolite and plant promoting activity in tomatoes seed 10:30 10:45 Miroslav Urosevic The importance of biosecurity measures in cyprinid fish ponds: the preliminary results from Bosnia and Herzegovina 10:45 11:00 Éva Laslo Foodborne Pathogens in Cheese: Their Origins, Risks, and One Health Relevance 11:00 11:15 Ali Aljeratly Multi-Element Profiling and Health Risk Assessment of Commercial Tahini Products using ICP-OES Lunchbreak 11:15 12:00 Regular talks 12:00 12:15 Food safety and foodborne diseases Károly Pál László Gyenge Investigation of the soaking of matured semi-hard cheeses in blackcurrant wine or in natural blackcurrant juice 12:15 12:30 Krisztina Pócsik-Sáfrány Risk factors for spinach consumption and how to improve them 12:30 12:45 Eyesun Naammo Effect of Chitosan Coating on Healthy and Infected Walnuts Break 12:45 13:00 Regular talks 13:00 13:15 Food safety and foodborne diseases Gyula Kasza Cristina Popovici Designing novel plant-based cheese-like product using walnuts: insights from color and texture parameters 13:15 13:30 Cristina Popovici Plant-based yoghurt: a promising sustainable alternative to dairy products 13:30 13:45 Roland Sipos Using management approaches and plant-based feed supplements in large-scale pig farms as alternative solutions to weaning ... 13:45 14:00 András Bittsánszky Effectiveness of food safety self-checking systems in Hungarian school catering 14:00 14:15 Zsuzsa Farkas CONTEMFOOD Project: Contaminants in Meat Replacement Products: A Contemporary Food Safety Perspective 14:15 14:30 Arthur Muhoro Recent research efforts on the detection of agrochemicals in honey and honey products in Kenya. 14:30 14:45 Gyöngyvér Boglárka Péterffy Evaluating the Antimicrobial Properties of Purslane (Portulaca oleracea L.) Extracts Against Clinically Relevant Pathogens 14:45 15:00 Loránd Alexa Pseudocereals in beer production: Investigation of quality, nutritional value and chemical safety Day 3 2025.06.20. Semi-plenary 8:30 9:00 Alternatives to antimicrobials in human and veterinary medicine Gábor Kardos Lars Fieseler Bacteriophages for the control of the fire blight pathogen Erwinia amylovora Regular talks 9:00 9:15 Marcell Gulyás Bacteriophages against antibiotic-resistant Klebsiella pneumoniae strains 9:15 9:30 Evelin Szoták Isolation and characterization of Stenotrophomonas maltophilia infecting bacteriophages Break 9:30 9:45 Regular talks 9:45 10:00 Social perceptions of antibiotics and their alternatives Éva Kertész Gergely Pethő AI-Supported Discourse Analysis of Vaccine Skepticism in a Post-Truth Context 10:00 10:15 Ágnes Hajdu Behavioural Drivers of Hand Hygiene: Early Results from the EU JAMRAI 2 WP7.4 Hungarian Pilot Study Using the COM-B Model... 10:15 10:30 Éva Libicki Left Behind in Health: The Consequences of Digital Disconnection and Limited Support 10:30 10:45 Flórián Sipos Towards Responsible Antibiotic Use: Progress Report on a Participatory Research Project in Primary Care 10:45 11:00 Gerda Holló Sequencing method optimized for monitoring microbiome changes in burn patients 11:00 11:15 Delia Costache Anaphylactic reaction secondary to surgically treated hydatid cyst: a case report 11:15 11:30 Conference closing Gábor Kardos Concluding remarks Lunchbreak 11:30 12:30 Breakout rooms 12:30 13:30 Breakout rooms on the following topics: - Current and future directions in phage research and applications - Future infectious threats: the need for preparedness - Veterinary science as an integral part of One Health