Abstract Knowledge sharing in healthcare is crucial for enhancing clinical practices and patient care. This study investigates the impact of work culture and the use of digital information resources on knowledge sharing among health professionals in state-owned hospitals in Oyo State, Nigeria. Employing a survey research design, the study sampled 234 out of 596 health professionals using a validated questionnaire with a 100% response rate. The findings indicate a high level of knowledge sharing, with an overall mean score of 3.33 (SD = 0.598) on a 4-point scale. Health professionals actively share knowledge on medical procedures, drug administration, best practices, and digital health technologies. Face-to-face interactions and mobile phones are the predominant channels for sharing, with 100% utilization reported. Despite moderate engagement with digital resources (M = 3.01, SD = 0.768), barriers such as inadequate technology use and lack of incentives affect knowledge sharing. Work culture significantly influences knowledge sharing, accounting for 55.6% of the variance, with hierarchical structures and criteria for success having positive effects. Digital resources use has a minimal influence (Adj. R² = 0.069). The study concludes that fostering a supportive work culture and enhancing digital resource accessibility are essential for improving knowledge sharing practices. It recommends frequent seminars and workshops and the establishment of online messaging platforms to facilitate continuous knowledge exchange. KEYWORDS: Work Culture, Digital Information Resources, Knowledge Sharing, Hospitals