Abstract Title: A comparative study to evaluate the accuracy of open tray splinted and non-splinted implant level impressions and impressions obtained from two intraoral scanners for three implants placed in a partially edentulous arch - an in vitro study Objective: The aim of this in vitro study was to compare and evaluate the accuracy of open tray splinted and non-splinted Implant level impression and impressions obtained from two intraoral scanners for three implants placed in a partially edentulous arch and to evaluate which of the two scanners used (Trios 3 shape and Upsera intraoral scanners) has the better trueness and precision value. Methods: Three implants were placed in a partially edentulous arch model simulating clinical conditions. Impressions were made using open tray splinted and non-splinted techniques and models were fabricated as well as the study model was scanned with two intraoral scanners (Trios 3 shape and Upsera intraoral scanners). The models were then digitally scanned and compared with a reference scan using a Exocad software. Measurements were made by superimposing the group scans over the reference scan. Results: The results revealed that the open tray splinted impression technique was found more accurate than the non-splinted impression techniques. However, impressions obtained from trios 3 shape intraoral scanners showed significantly higher accuracy compared to the other scanner and the conventional impression techniques. The differences in accuracy were statistically significant (p < 0.05) at specific landmarks. Conclusion: Within the limitations of this study, it can be concluded that open tray splinted was superior than non-splinted impression techniques in terms of accuracy for three implants placed in a partially edentulous arch. However, trios 3 shape intraoral scanners demonstrated superior accuracy compared to Upsera intraoral scanner and conventional impression techniques.