This dataset comprises simulated DNA sequencing reads in FASTQ format for 17,900 complete NCBI RefSeq virus sequences downloaded on 2025-02-05. Included are simulated long Oxford Nanopore Technologies R10.4 reads with ~4% error rate and simulated short (2x150bp) Illumina reads with 1% error rate. The source reference sequences are provided as rsviruses17900.fa.gz. Simulated long reads (Oxford Nanopore Technologies) rsviruses17900.fastq.gz Measured empirical error rate: ~4% Simulator: PBSIM 3.0.4 (https://academic.oup.com/nargab/article/4/4/lqac092/6855700) Model: ERRHMM-ONT-HQ Depth: 10x Mean read length: 1,000bp Max read length: 10,000bp Mean accuracy: 0.98 Random seed: 1 Command used: for fasta in rsviruses17900/*.fa; do acc=$(basename "$fasta" .fa) pbsim --seed 1 --strategy wgs --method errhmm --errhmm pbsim3/data/ERRHMM-ONT-HQ.model --depth 10 --genome ${fasta} --prefix ${acc} --id-prefix ${acc}__ --length-mean 1000 --length-max 10000 --accuracy-mean 0.98; cat ${acc}*.fastq | pigz > ${acc}.fastq.gzdone Simulated short reads (Illumina) rsviruses17900.r1.fastq.gz and rsviruses17900.r2.fastq.gz Measured empirical error rate: 1% Simulator: dwgsim 0.1.14; conda package version 1.1.14, (https://github.com/nh13/DWGSIM) Read length: 2x150bp (paired) Depth: 10x Random read probability (-y): 0 Error rate (-e and -E): 0.01 Mutation rate (-r): 0.0 Of which low frequency somatic mutations (-F): 0.0 Random seed (-z): 1 Command used: for fasta in rsviruses17900/*.fa; do acc=$(basename "$fasta" .fa) dwgsim -C 10 -1 150 -2 150 -y 0.0 -o 1 -z 1 -F 0.0 -r 0.0 -e 0.01 -E 0.01 "$fasta" "$acc"done