A guide to selecting high-performing antibodies for Rab7A (UniProt ID: P51149) for use in western blot, immunoprecipitation, and immunofluorescence
A guide to selecting high-performing antibodies for Rab7A (UniProt ID: P51149) for use in western blot, immunoprecipitation, and immunofluorescence
This report presents a guide to selecting high-quality commercial antibodies against GTPase Rab7A in western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic controls. This work was supported by the CQDM (by a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec) as well as the Government of Canada through Genome Canada, Genome Quebec, and Ontario Genomics (grant no. OGI-210).
Ras-related protein Rab-7a, antibody characterization, western blot, RAB7A, Rab7A, antibody validation, immunofluorescence, immunoprecipitation, P51149
Ras-related protein Rab-7a, antibody characterization, western blot, RAB7A, Rab7A, antibody validation, immunofluorescence, immunoprecipitation, P51149
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