The dataset includes twenty-eight (28) Fourier transform infrared (FTIR) spectroscopy with attenuated total reflectance (ATR) spectral data in a CSV file of Latvian honey samples: Clover honey (Trifolium repens, code: Clo) - 3 samples; Linden honey (Tilia cordata, code: Lin) - 4 samples. Willow honey (Salix cinerea, code: Wil) - 4 samples. Rapeseed honey (Brassica napus, code: Rap) - 2 samples, Buckwheat honey (Fagopyrum esculentum, code: Buck) - 5 samples. Heather honey (Calluna vulgaris, code: Hea) - 3 samples, Apiaceae honey (Apiaceae sp., code: Api) - 2 samples and Raspberry honey (Rubus idaeus, code: Ras) – 1 sample. Spectra of Latvian honey samples were compared with commercial Manuka honey (Produced by Oha Honey LP (Masterton, New Zealand). Attenuated total reflection (ATR) spectroscopy spectra of all honey samples were taken with Thermo Fisher Scientific Nicolet iS5 with a diamond crystal. Spectra were recorded from 500 to 4000 cm−1 with 32 scans and a resolution of 4 cm−1, optical velocity of 0.4747, and aperture of 100%. The sample names correspond to those used in the publication: Skadiņš, I.; Labsvārds, K.D.; Grava, A.; Amirian, J.; Tomsone, L.E.; Ruško, J.; Viksna, A.; Bandere, D.; Brangule, A. Antimicrobial and Antibiofilm Properties of Latvian Honey against Causative Agents of Wound Infections. Antibiotics 2023, 12, 816. https://doi.org/10.3390/antibiotics12050816 Acknowledgments The authors acknowledge the access to the infrastructure and expertise of the BBCE—Baltic Biomaterials Centre of Excellence (European Union’s Horizon 2020 research and innovation programme under the grant agreement No. 857287) and “Strengthening of the capacity of doctoral studies at the University of Latvia within the framework of the new doctoral model”, identification no. 8.2.2.0/20/I/006. Created: 2023-02-02