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Chromatogram alignments of white lupin genome contigs and PCR products obtained for PR_74, PR_76, PR_77, PR_78, PR_79 and PR_80 markers using DNA isolated from representative white lupin lines

Name: Chromatogram alignments of white lupin genome contigs and PCR products obtained for PR_74, PR_76, PR_77, PR_78, PR_79 and PR_80 markers using DNA isolated from representative white lupin lines
Keywords: flowering, PCR, Flowering locus T

appsOther research productkeyboard_double_arrow_right Other ORP type 15 Apr 2025 English Publisher:Zenodo

Authors: Bielski, Wojciech Krzysztof; Surma, Anna Maria; Książkiewicz, Michał; Rychel-Bielska, Sandra;

doi: 10.5281/zenodo.15223456 , 10.5281/zenodo.15223455

Chromatogram alignments of white lupin genome contigs and PCR products obtained for PR_74, PR_76, PR_77, PR_78, PR_79 and PR_80 markers using DNA isolated from representative white lupin lines

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Abstract

PR_74 marker White lupin pangenome contigs: LD37_contig_002595 (white lupin line LD37) P27174-4_contig_000651 (white lupin line P27174) LalbFTa1_Chr02 white lupin line Amiga) White lupin PCR products: PR74-24_PR74-F.ab1 (forward): primer PRFTa1_F1c, white lupin line “24”, accession number “95187”, name “BGRC-3911”, single product PR74-119c_PR74-F.ab1 (forward): primer PRFTa1_F1c, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product PR74-24_PR74-R.ab1 (reversed): primer PRFT1a_F2aR0, white lupin line “24”, accession number “95187”, name “BGRC-3911”, single product PR74-119c_PR74-R.ab1 (reversed): primer PRFT1a_F2aR0, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product PR74-D_PR74-F.ab1 (forward): primer PRFTa1_F1c, white lupin line “D”, accession number “95519”, name “P27174”, single product PR74-K_PR74-F.ab1 (forward): primer PRFTa1_F1c, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR74-D_PR74-R.ab1 (reversed): primer PRFT1a_F2aR0, white lupin line “D”, accession number “95519”, name “P27174”, single product PR74-K_PR74-R.ab1 (reversed): primer PRFT1a_F2aR0, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR_76 marker White lupin pangenome contigs: LD37_contig_002595_: (white lupin line LD37) P27174-4_contig_: (white lupin line P27174) LalbFTa1_Chr02_: (white lupin line Amiga) White lupin PCR products:PR76-19-3_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “19”, accession number “95149”, name “AL-22”,the shortest product PR76-14-3_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “14”, accession number “95130”, name “Population-84152”, the shortest product PR76-14-3_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “14”, accession number “95130”, name “Population-84152”, the shortest product PR76-19-3_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “19”, accession number “95149”, name “AL-22”, the shortest product PR76-D_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “D”, accession number “95519”, name “P27174”, single product PR76-K_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR76-K_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR76-D_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “D”, accession number “95519”, name “P27174”, single product PR76-24_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “24”, accession number “95187”, name “BGRC-3911”, single product PR76-119c_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product PR76-24_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “24”, accession number “95187”, name “BGRC-3911”, single product PR76-119c_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product PR76-19-2_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “19”, accession number “95149”, name “AL-22”, intermediate product PR76-19-2_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “19”, accession number “95149”, name “AL-22”, intermediate product PR76-14-2_PR76-R.ab1 (reversed): primer PRFTa1_R2b, white lupin line “14”, accession number “95130”, name “Population-84152”, intermediate product PR76-14-2_PR76-F.ab1 (forward): primer PRFTa1_F2a2, white lupin line “14”, accession number “95130”, name “Population-84152”, intermediate product PR_77 marker White lupin pangenome contigs: GR38_contig_001073: (white lupin line GR38) LalbFTa2_Chr21: (white lupin line Amiga) White lupin PCR products: PR77-17c_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “LAP017c”, accession number “LAP017c”, name “Gr33”, single product PR77-17d_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “LAP017d”, accession number “LAP017d”, name “Gr33”, single product PR77-17d_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “LAP017d”, accession number “LAP017d”, name “Gr33”, single product PR77-17c_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “LAP017c”, accession number “LAP017c”, name “Gr33”, single product PR77-64-1_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “64”, accession number “95602”, name “Population-775”, longer product PR77-64-1_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “64”, accession number “95602”, name “Population-775”, longer product PR77-26c_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “LAP026c”, accession number “LAP026c”, name “Tr21”, single product PR77-26c_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “LAP026c”, accession number “LAP026c”, name “Tr21”, single product PR77-65_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “65”, accession number “95605”, name “Population-743”, single product PR77-65_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “65”, accession number “95605”, name “Population-743”, single product PR77-K_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR77-K_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR77-D_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “D”, accession number “95519”, name “P27174”, single product PR77-D_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “D”, accession number “95519”, name “P27174”, single product PR77-64-2_PR77-R.ab1 (reversed): primer PRFTA2R4, white lupin line “64”, accession number “95602”, name “Population-775”, shorter product PR77-64-2_PR77-F.ab1 (forward): primer PRFTA2F5, white lupin line “64”, accession number “95602”, name “Population-775”, shorter product PR_78 marker White lupin pangenome contigs: GR38_contig_001073: (white lupin line GR38) LalbFTa2_Chr21: (white lupin line Amiga) White lupin PCR products: PR78-D_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “D”, accession number “95519”, name “P27174”, single product PR78-D_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “D”, accession number “95519”, name “P27174”, single product PR78-K_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR78-K_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR78-64-3_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “64”, accession number “95602”, name “Population-775”, the shortest product PR78-64-3_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “64”, accession number “95602”, name “Population-775”, the shortest product PR78-115c-3_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, the shortest product PR78-115c-3_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, the shortest product PR78-17c_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “LAP017c”, accession number “LAP017c”, name “Gr33”, single product PR78-65_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “65”, accession number “95605”, name “Population-743”, single product PR78-17c_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “LAP017c”, accession number “LAP017c”, name “Gr33”, single product PR78-65_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “65”, accession number “95605”, name “Population-743”, single product PR78-64-2_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “64”, accession number “95602”, name “Population-775”, intermediate product PR78-64-2_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “64”, accession number “95602”, name “Population-775”, intermediate product PR78-115c-2_PR78-R.ab1 (reversed): primer PRFTA2R6, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, intermediate product PR78-115c-2_PR78-F.ab1 (forward): primer PRFTA2F6, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, intermediate product PR_79 marker White lupin pangenome contigs: Clovis_contig_006417: (white lupin line Clovis) LalbFTa2_Chr21: (white lupin line Amiga) White lupin PCR products: PR79-D_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “D”, accession number “95519”, name “P27174”, single product PR79-K_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR79-K_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR79-D_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “D”, accession number “95519”, name “P27174”, single product PR79-115c-3_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, the shortest product PR79-48-3_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “48”, accession number “95468”, name “Azurnyj”, the shortest product PR79-115c-3_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, the shortest product PR79-119c_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product PR79-119d_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “LAP119d”, accession number “LAP119c”, name “Aster”, single product PR79-119c_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “LAP119c”, accession number “LAP119c”, name “Aster”, single product PR79-119d_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “LAP119d”, accession number “LAP119c”, name “Aster”, single product PR79-115c-2_PR79-R.ab1 (reversed): primer PRFTA2_R7, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, intermediate product PR79-115c-2_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “LAP115c”, accession number “LAP115c”, name “Energy”, intermediate product PR79-48-2_PR79-F.ab1 (forward): primer PRFTA2_F7, white lupin line “48”, accession number “95468”, name “Azurnyj”, intermediate product PR_80_marker White lupin pangenome contigs: LD37_contig_001630: (white lupin line LD37) P27174-4_contig_001786: (white lupin line P27174) LalbFTc1_Chr14: (white lupin line Amiga) GR38_contig_001107: (white lupin line GR38) White lupin PCR products: PR80-18d_PR80-F.ab1 (forward): primer PRFTc1_F2e, white lupin line “LAP018d”, accession number “LAP018d”, name “Gr49”, single product PR80-18d_PR80-R.ab1 (reversed): primer PRFTc1_R2e, white lupin line “LAP018d”, accession number “LAP018d”, name “Gr49”, single product PR80-22e_PR80-F.ab1 (forward): primer PRFTc1_F2e, white lupin line “LAP022e”, accession number “LAP022e”, name “Tr7”, single product PR80-22e_PR80-R.ab1 (reversed): primer PRFTc1_R2e, white lupin line “LAP022e”, accession number “LAP022e”, name “Tr7”, single product PR80-D_PR80-F.ab1 (forward): primer PRFTc1_F2e, white lupin line “D”, accession number “95519”, name “P27174”, single product PR80-D_PR80-R.ab1 (reversed): primer PRFTc1_R2e, white lupin line “D”, accession number “95519”, name “P27174”, single product PR80-K_PR80-F.ab1 (forward): primer PRFTc1_F2e, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product PR80-K_PR80-R.ab1 (reversed): primer PRFTc1_R2e, white lupin line “K”, accession number “95479”, name “Kiev Mutant”, single product

Related Organizations

University of Life Sciences in Poznań
Poland
Polish Academy of Sciences
Poland
Institute of Plant Genetics
Poland
Wroclaw University of Environmental and Life Sciences
Poland

Keywords

flowering, PCR, Flowering locus T

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