21. Amblyomma triste Koch, 1844. ND: ND, ND (Woodham et al. 1983; Guzmán-Cornejo et al. 2011). San Fernando: ND, B. taurus (CFPP 2015, 2016). Soto la Marina: ND, B. taurus (CFPP 2015, 2016). Distribution: Tamaulipas Province and Veracruzan Province. Note: Amblyomma triste and A. maculatum belong to the group known as Amblyomma maculatum (Camicas et al. 1998). The validity of these species has been questioned and analyzed morphologically and molecularly. Lado et al. (2018) suggested that A. triste should be synonymized with A. maculatum, although they mentioned the need to use new markers and crossbreeding experiments. On the other hand, Ossa-López et al. (2024) gave new evidence for the validity of A. triste, by comparing the mitochondrial genome of A. triste and A. maculatum obtaining a difference of 4.2%. However, these authors also pointed out the need for sequencing the mitochondrial genome of A. tigrinum, which is part of the A. maculatum group, as well as experimental crosses of populations from different places in the Americas to obtain additional evidence for species recognition.

Published as part of Rodríguez-García, Iram Emmanuel, Coronado-Blanco, Juana María, Garrido-Olvera, Lorena, Guzmán-Cornejo, Carmen, López-Aguirre, Daniel, Estrada-Drouaillet, Benigno, Niño-Maldonado, Santiago & Guerra-Pérez, Antonio, 2025, Updated list of tick species (Parasitiformes: Ixodida) in Tamaulipas: distribution and hosts, pp. 301-325 in Zootaxa 5618 (3) on page 307, DOI: 10.11646/zootaxa.5618.3.1, http://zenodo.org/record/15217941