This thesis examines the application of third-person pronouns in two distinct languages: English and Uzbek. The assessment centers on significant aspects such as gender differentiation, levels of formality, possessive forms, reflexive pronouns, and instances where pronouns are omitted in both languages. In English, third-person pronouns are specific to gender, featuring distinct forms for masculine, feminine, and neutral subjects, while a noticeable distinction between formal and informal address is conveyed through titles rather than pronouns. Conversely, Uzbek employs a neutral third-person pronoun that does not differentiate by gender, with formality articulated through particular honorifics and plural constructions. Moreover, the agglutinative nature of Uzbek permits the omission of pronouns when the subject is implied through verb conjugation, a feature absent in English. This paper underscores how these variations mirror the grammatical frameworks and cultural practices of each language, offering insights into the ways language influences social interactions and communication. These linguistic traits not only shape daily conversations but also have repercussions on literature, media, and education, illuminating the profound links between language, identity, and societal values. Grasping these subtleties is crucial for language learners and educators, as it cultivates a deeper understanding of the complexities of communication and promotes more effective cross-cultural exchanges.