Table 2. Primers and cycler programmes used for the molecular analysis. MarkerPrimer sequences and combinationReferenceCycler programmerITSITS5 5'-GGA AGT AAA AGT CGT AAC AAG G-3'White et al. (1990)95 °C for 5 min, 33 cycles of amplification (95 °C for 15 s, 55 °C for 30 s, 72 °C for 40 s), 72 °C for 5 minITS4 5'-TCC TCC GCT TAT TGA TAT GC-3'rbcL (partial)rbcLaF 5'- ATG TCA CCA CAA ACA GAG ACT AAA GC-3'Levin et al. (2003)95 °C for 5 min, 35 cycles of amplification (95 °C for 10 s, 55 °C for 30 s, 72 °C for 40 s), 72 °C for 5 minrbcLaR 5'-GTA AAA TCA AGT CCA CCR CG-3'Kress et al. (2009)atpB-rbcL spaceratpB-rbcL F 5'-GAA GTA GTA GGA TTG ATT CTC-3'Golenberg et al. (1993)95 °C for 5 min, 35 cycles of amplification (95 °C for 20 s, 56 °C for 30 s, 72 °C for 60 s), 95 °C for 20 s, 56 °C for 80 s, 72 °C for 8 minatpB-rbcL R 5'-CAA CAC TTG CTT TAG TCT CTG-3'trnL-FTab C 5'-CGA AAT CGG TAG ACG CTA CG-3'Taberlet et al. (1991)95 °C for 5 min, 35 cycles of amplification (95 C for 1 min, 50 °C - 65 °C (increasing in 0.3 C per cycle) for 1 min, 72 °C for 4 min), 72 °C for 5 minTab D 5'-GGG GAT AGA GGG ACT TGA AC-3'Tab E 5'- GGT TCA AGT CCC TCT ATC CCC-3'Tab F 5'ATI' TGA ACT GGT GAC ACG AG 3'

Published as part of Sukhorukov, Alexander P., Nilova, Maya V., Krinitsina, Anastasiya A., Maxim A. Zaika,, Erst, Andrey S. & Shepherd, Kelly A., 2018, Molecular phylogenetic data and seed coat anatomy resolve the generic position of some critical Chenopodioideae (Chenopodiaceae - Amaranthaceae) with reduced perianth segments, pp. 103 in PhytoKeys 109 on page 103, DOI: 10.3897/phytokeys.109.28956, http://zenodo.org/record/1476320