Table 1 Major products (%±SD) of WT α- pinene synthase and enzymes with mutations. EnzymesRelative Proportion of Products (%)Unknownα- pinenesabinenelimoneneUnknownUnknownActivity(% WT)RT = 4.52RT = 7.05RT = 8.55RT = 11.05RT = 11.58RT = 12.72PS-WT0.31 ± 0.0297.86 ± 0.210.96 ± 0.000.26 ± 0.010.54 ± 0.050.07 ± 0.00100F482A0.05 ± 0.004.43 ± 0.0393.21 ± 0.061.59 ± 0.050.72 ± 0.030.00 ± 0.0046.63 ± 4.47S491A0.46 ± 0.0197.28 ± 0.091.14 ± 0.000.12 ± 0.000.99 ± 0.060.00 ± 0.00128.90 ± 4.19F482I1.27 ± 0.023.17 ± 0.0892.35 ± 0.210.66 ± 0.092.33 ± 0.080.23 ± 0.0018.36 ± 2.51F482L0.96 ± 0.062.03 ± 0.0394.26 ± 0.300.31 ± 0.011.91 ± 0.170.29 ± 0.0149.98 ± 39.78F482V1.03 ± 0.012.12 ± 0.0394.51 ± 0.070.30 ± 0.031.82 ± 0.060.23 ± 0.0029.50 ± 12.39F482T0.00 ± 0.003.53 ± 0.0093.91 ± 0.001.64 ± 0.040.92 ± 0.050.00 ± 0.0052.69 ± 3.17F482Y0.002.7131.0063.612.680.000.62F482R17.62 ± 6.210.00 ± 0.0018.96 ± 7.1931.04 ± 19.2727.33 ± 14.945.05 ± 0.511.28 ± 0.01F482W16.32 ± 5.330.00 ± 0.0029.92 ± 17.9021.67 ± 6.8427.35 ± 14.964.74 ± 0.451.54 ± 0.02I335A3.52 ± 0.0278.00 ± 0.0312.94 ± 0.000.57 ± 0.024.28 ± 0.030.67 ± 0.0054.36 ± 2.17F482A/S491A1.34 ± 0.114.17 ± 0.0188.40 ± 1.433.38 ± 0.142.36 ± 0.340.35 ± 0.0118.73 ± 0.13F482I/S491A0.63 ± 0.022.72 ± 0.0092.88 ± 0.452.40 ± 0.091.19 ± 0.080.18 ± 0.0028.74 ± 5.73

Published as part of Xu, Jingwei, Peng, Guanzu, Xu, Jinkun, Li, Yi, Tong, Li & Yang, Dong, 2021, Probing of the plasticity of the active site in pinene synthase elucidates its potential evolutionary mechanism, pp. 3 in Phytochemistry (112573) 181 on page 3, DOI: 10.1016/j.phytochem.2020.112573, http://zenodo.org/record/8291066