An experiment was conducted during the year 2017-18 to investigate the vernalization duration and photo period effect on growth and flowering response of pansy (Viola tricolor L.). The research was done at Horticulture Garden, Sindh Agriculture University Tandojam. The experimental design was factorial in Completely Randomized Design (CRD) replicated thrice. The bulbs of pansy were kept in different vernalization durations as 02 days at 10oC, 04 days at 10oC, 06 days at 10oC and untreated bulbs were treated as control. Vernalization treated bulbs were planted in earthen pots and kept in tree shade and full sun. Data were observed for days to sprouting, height of the plant in cm, plant spread (cm2), no of leaves-plant-1, days toflower -persistence, no flowers-plant-1, weight of single flower(g), flower diameter (cm) and days to flowering initiation. The growth and flowering characteristics of pansy were significantly affected by vernalization treatments and growing conditions. Result revealed that the vernalization duration 02 days at 10oC significantly affected for plant height (6.27 cm), plant spread (9.67 cm2), leaves per plant(18.00), days to flower-persistence (4.75) and flower diameter (5.97 cm). Earlier flower initiation (117.00) was found at vernalization duration 02 days at 10 ºC. While respect to growing conditions Days to sprouting (9.37) was earlier where bulbs are kept in tree shade and tree shade had superior results for plant height (6.48 cm), plant spread (9.6 cm2), leaves per plant(17.33), days to flower-persistence (7.99), number of flowers plant-1 (13.66) and flower diameter (5.17 cm). It is concluded that pansy bulbs had better results for growth and flowering characteristics at vernalization duration 02 days at 10oC. Whereas “tree shade” responded a significant better growth and the parameters for flowering as compared to “full sun”.