The rise of 24-hour retail stores in urban and semi-urban areas reflects the growing demand for convenience. This study examines the challenges and opportunities faced by these establishments in Batangas, Philippines, using a qualitative case study approach. Semi-structured interviews with store owners and employees reveal key operational, logistical, and regulatory difficulties. Operational challenges include inventory mismanagement, staffing shortages, and fluctuating sales. Poor stock rotation and inaccurate demand forecasting result in expired products and financial losses. Staffing, particularly for night shifts, is problematic due to high turnover and scheduling conflicts, affecting service quality. Seasonal demand fluctuations add further strain, making revenue unpredictable while operational costs remain fixed. Logistical challenges involve supply chain inefficiencies, security concerns, and inventory shortages. Dependence on external suppliers often leads to delivery delays, while theft and vandalism risks require improved security measures. Regulatory compliance adds complexity, particularly for small businesses struggling with labor laws, fair compensation, and workplace safety. Despite these challenges, opportunities for growth exist. The 24-hour model attracts customers with non-traditional schedules, such as students and night-shift workers. Personalized customer service, loyalty programs, and targeted promotions enhance engagement and retention. Technological innovations, such as automated inventory systems and digital payments, improve efficiency. To remain competitive, stores must improve inventory management, adopt flexible staffing strategies, strengthen supply chain partnerships, and enhance security. Addressing these challenges while leveraging growth opportunities ensures sustainability and long-term success in the evolving retail landscape.