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USP5 Expression and Purification & Development of a Mass Spectrometry USP5 Catalytic Activity Assay

Research datakeyboard_double_arrow_right Dataset 23 Nov 2018Publisher:Zenodo

Authors: Mann, Mandeep; Ackloo, Suzanne; Harding, Rachel; Edwards, Aled; Arrowsmith, Cheryl; Schapira, Matthieu;

doi: 10.5281/zenodo.1495189 , 10.5281/zenodo.1495188

USP5 Expression and Purification & Development of a Mass Spectrometry USP5 Catalytic Activity Assay

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Abstract

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

Expression and purification of full length USP51-835 for optimization of a mass spectrometry (MS) based assay to detect polyubiquitin cleavage

Related Organizations

University of Toronto
Canada

Keywords

zinc finger, USP5, protein purification, catalytic activity, mass spectrometry

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