Abstract Artificial Intelligence (AI) is transforming biblical research and interpretation by offering advanced tools for textual analysis, translation studies and historical context evaluation. This study explores the role of AI in enhancing biblical scholarship, focusing on its applications in exegesis, language studies and hermeneutics. AI-driven tools assist scholars by automating research processes, improving textual comparisons and aiding in historical and linguistic analyses. However, ethical concerns arise, including biases in AI models, the risk of over- reliance on technology and the potential diminishment of spiritual discernment. The study also examines the challenges and limitations of AI in biblical studies, particularly its dependence on historical-critical methods and ethical dilemmas in AI training. Despite these challenges, AI contributes positively to biblical scholarship by increasing research efficiency, accessibility and accuracy. The intersection of technology, theology and biblical interpretation requires careful navigation to balance faith with digital advancements. The study emphasizes the importance of human oversight, ethical AI development and increased accessibility of AI tools. Recommendations include training scholars in AI literacy, promoting balanced use of AI in biblical research and ensuring AI tools align with theological integrity. This research concludes that AI is a valuable complement to traditional biblical studies but should not replace human exegesis and spiritual discernment. (Colossians 2:8; Proverbs 4:7). Keywords: Artificial Intelligence, Exegesis, Hermeneutics, Textual Analysis and Natural Language Processing