The determination of metal ions such as Cu²⁺ and Zn²⁺ in various samples is crucial for environmental monitoring, industrial quality control, and biomedical applications. This study explores the quantitative analysis of Cu²⁺ and Zn²⁺ ions using UV/Vis spectrophotometry, a rapid and reliable technique based on the absorption of light by metal-ligand complexes. Optimal conditions for the detection, including wavelength selection, reagent concentration, and pH, were investigated to enhance sensitivity and accuracy. Calibration curves were constructed to determine the linearity and detection limits for each ion. The method was applied to real samples, and results were validated against standard techniques. The findings demonstrate that UV/Vis spectrophotometry provides a cost-effective and efficient approach for Cu²⁺ and Zn²⁺ determination in various matrices.