The physical properties of bone tissue have been investigated at different levels, macro, micro and nano scale. The aim of this study was to assess the influence of two specimen preparation techniques and six nanoindentation protocols on modulus and hardness of bovine bone specimens. Embedding in resin do not affect the mechanical properties of bone samples. More precise and repeatable results are obtained using higher indentation forces. Larger number of measurements are required for bone indentation analysis using lower forces.