The main characteristic of the electrochemically activated aqueous solutions" (ECAAS) is that if they are subjected to unipolar electrochemical influences with certain characteristics, in them occur metastable, i. g. electrochemically activated substances giving them unique ecological, chemical, physical, biological and, in particular, microbiological properties, which all the currently authorized chemicals (biocides, cosmetics and pharmaceuticals) do not possess. The anolyte and has reduced electron activity and pronounced oxidant properties, and the catholyte has enhanced electron activity and reductor properties. Because of their low oxidant content, the anolytes have very little chemical buffering, hence their environmental safety. The qualities of the ECAAS have provided grounds for their broad application both in the human and in the veterinary field, in the military field, the food industry, for the disinfection of ships, wagons, airplanes, etc. We conducted tests to determine the effect of an anolyte containing Cl- obtained by electrochemical activation of sterile distilled water with 3% NaCl on depleted sludge from a municipal wastewater treatment plant, on bovine manure and on Clostridium perfringens. The results show that the anolyte added in equal proportion to the manure resulted in a reduction in the total number of microorganisms 20 times (2.6x105 CFU/ml) compared to the untreated control manure (7.5x106 CFU/ml), and upon addition of a doubly lesser amount of anolyte, the total number of microorganisms decreased 10 fold (4.5x105 CFU/ml) relative to the control. After 24 hours influence of anolyte on depleted sludge, there was a decreasing in the amount of microorganisms from all the groups studied. The decreasing was statistically significant in the total number of microorganisms and that of the Gram-negative aerobes in comparison with the untreated sludge, more significant in the sludge treated with equal quantity anolyte than that, treated with twice smaller amount of anolyte. The anolyte added in equal amounts to a suspension of C. perfringens at a density of 2.0x106 CFU/ml after 10 minutes of exposure, caused a three-fold decrease in the amount of viable cells in the suspension.