Abstract Tuberculosis is a highly infectious illness in Pakistan. The aim of this study was to assess the knowledge and practice of nurses in the prevention and control of tuberculosis infections among nurses working in teaching hospital, Lahore. This cross-sectional study involved the participation of nurses from all sections and outpatient departments at Govt. Said Mitha Hospital, Lahore, and Sir Ganga Ram Hospital Lahore. Data was collected from (n=156) nurses working in each ward and outpatient department of the hospitals using a semi-structured and self-administered survey. Findings revealed that a significant proportion of nurses showed a deficiency in knowledge and used insufficient strategies for the prevention and control of tuberculosis. All nurses reported using a chemical disinfectant called Savlone / chlorine solution to cleanse the room and its surfaces. Nurses who were older than and those who were working in govt. Gangaram hospital Lahore at the time had a higher probability of being aware of TBIPC. Results showed that the training is needed to follow the correct protocols for preventing and controlling TB. That makes them more likely to control tuberculosis. In conclusion, the hospital must ensure the health of the nurses who provide tuberculosis patient care. Keywords: Knowledge and Practice, Prevention and Control, Tuberculosis, Nurses, Teaching Hospital, Lahore