Background: Breast cancer is a malignant tumor that begins in the breast cells . Malignant tumor is a group of cancer cells that able to grow into surrounding tissues or spread (metastasize) to faraway regions of the body. The disease take place nearly fully in women ,but men can get it, also. Malondialdehyde (MDA) is end-products of lipid hydroperoxide decomposition and one of many low molecular weight and is the mostly measured as an indicator of lipid peroxidation . Aim: This study aims to investigate the relation between Malondialdehyde (MDA) and nitric oxide (NO level with the development of Breast Cancer. Methods: Plasma of Malondialdehyde (MDA) and nitric oxide (NO) were determined in 46 patients with Breast cancer and 21 healthy subjects as control group using by the colorimetric method,. All results were statistically analyzed. Results: A highly significant increase was found in the serum level MDA in patients with Breast Cancer compared to control (P < 0.05). serum levels of NO were significantly increased in the patient group (P < 0.05) compared to control. Conclusion: The results of the present study provide evidence that the family history has a clear link with breast cancer risk while does not have such a link with smoking or lodging. High levels of oxidative/nitrosative stress statues were presented in patients with breast cancer.