Reads obtained from DNA sequencing of two strains resulting from the horizontal chromosome transfer experiment described in [doi: 10.1038/nature08850] and submitted to ENA [PRJEB29294] . For each strains two paired-end libraries were generated, one with ifragment length of about 170 bp and one with fragmentlength of 500 bp. They were first mapped to the genome of Fo47, unmapped reads were subsequently mapped to the genome of Fol4287, as described in [doi: 10.1101/465070]. Putative PCR duplicates were removed using Picard tools and resulting bamfiles (one for the 170 fragment library and one for the 500 bp fragment library) were merged. These files are the resulting bamfiles. Fo1A_L170_L500.unmapped_to_Fo47illumina.Fol4287broad.bowtie2.sorted_dedup.bam (one chromosome: chr 14) Fo2A_L170_L500.unmapped_to_Fo47illumina.Fol4287broad.bowtie2.sorted_dedup.bam (two chromosomes: chr 14 + small chromosome)