In previous investigations, we reported that pre-exposure to radiofrequency (RF) withdifferent frequencies, modulation patterns, and specific absorption rate (SAR) levels, canreduce the damage induced by physical and chemical agents in different cellmodels. This study carried out within the NextGEM project funded by the EuropeanUnion, concerns the evaluation of the non-thermal effects of exposure and co-exposureof human neuroblastoma cells (SH-SY5Y) to RF, in terms of ROS formation, apoptosis,and cell cycle progression.SH-SY5Y cells were exposed for three hours to 1950 MHz, LTE signal at SAR levels of 0.3and 1.25 W/kg, and co-exposed to menadione, a well-known cytotoxic chemical agent.To date, the effect on ROS formation has been carried out by flow cytometer. Thepreliminary results here reported indicate: i) no effect on ROS formation after exposureto RF alone at both SAR levels investigated, ii) induction of ROS formation by MDtreatments as a function of MD concentration, and iii) no effect of combined exposure ofRF with 20 μM MD. Subsequent investigations will be carried out to consolidate theseresults and to assess the effects on apoptosis and cell cycle.