Investigating zinc oxide nanoparticles (ZnO NPs) and their biological manufacturing techniques has been fueled by the cosmetics industry's search for novel and sustainable solutions. This thorough analysis explores the changing field of ZnO NPs, emphasizing their biological production and several cosmetic uses. After providing some background information on the limits of conventional chemical synthesis, we explore the wide range of biological agents—such as bacteria, fungi, and plants—that are used to synthesize ZnO NP. The distinct physicochemical characteristics of organically manufactured ZnO NPs are revealed by characterization approaches, demonstrating their potential benefits over their conventionally produced counterparts. Understanding the effectiveness of these nanoparticles in cosmetic formulations is made possible by the ensuing investigation of their characteristics. ZnO NPs are used in sunscreens, skincare products, and other formulations in the cosmetics industry; their biological production gives them unique properties. The responsible incorporation of these nanoparticles into cosmetic products is discussed, along with safety and regulatory issues. The review provides a roadmap for furthering research in biological synthesis techniques and cosmetic applications by identifying present issues and speculating about future approaches. Successful uses are demonstrated by case studies, which provide a concrete viewpoint on incorporating biologically produced ZnO NPs into cosmetic formulations. In summary, this analysis highlights the critical role that ZnO NPs will play in determining the direction of the cosmetics sector and compiles the most recent developments in their biological synthesis. Researchers, industry professionals, and regulatory bodies can benefit greatly from this paper's comprehensive approach, which covers synthesis mechanisms, characterization, applications, safety issues, and prospects.